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A bacterial artificial chromosome (BAC), RPCI23-385H16, was used to isolate 144kb sequence containing the entire mouse Prox1 (prospero-related homeobox 1) gene. A 144kb portion of this BAC, containing only Prox1, was modified to include a red fluorescent protein, tdTomato, from the pClasper vector, between amino acid 5 and the Prox1 nuclear localization sequence.

Sep 09, 2013